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Application Notes

Luminescence/Chemiluminescence

HitHunter™ cAMP HS+ assay with Mithras LB 940
PathHunter™ ß-arrestin assay with Mithras LB 940
PathHunter™ ß-arrestin flash assay with Mithras LB 940
PathHunter™ secretion assay with Mithras LB 940

Fluorescence

Neutrophil Chemotaxis Assay performed on Twinkle LB 970
Cell viability assays with Mithras LB 940
Fura Calcium monitoring with the Mithras LB 940 multimode plate reader
ADP Quest™ HS kinetic assay with Mithras LB 940

Absorbance

PhosphoQuest™ cell based ELISA kinase assay with Mithras LB 940
NEW - TRANSIL® Brain Absorption Assay with Mithras LB 940

Bioluminescence Resonance Energy Transfer (BRET)

Comparison of filter sets for BRET1 assays: beta-arrestin2 recruitment to the vasopressin V2 receptor
A Functional BRET Assay for 7TM Receptors Using the Mithras LB 940 Multimode Plate Reader
Bioluminescence Resonance Energy Transfer (BRET)-based studies of receptor dynamics in living cells with Berthold’s Mithras
Bioluminescence Resonance Energy Transfer (BRET) as a means of monitoring dynamic receptor-protein interactions in living cells measured on LB 940 Mithras
Basic Considerations for Bioluminescence Resonance Energy Transfer (BRET) Assays for G-protein coupled receptor protein interactions in living cells

HTRF® (Homogeneous time-resolved fluorescence)

cAMP/IP-One HTplex cell-based experiment performed on Mithras LB940 using HTRF® technology

Fluorescence Polarisation (FP)

Characterization of the interaction between recombinant estrogen receptor alpha and transcriptional coactivator SCR-1 NR-2 binding motif through fluorescence polarization study with Mithras LB 940

Alpha Screen®

Mithras LB 940 Multimode Plate Reader Used for AlphaScreen Signal Detection: Measurement of cAMP Production Induced by a G-protein Coupled Receptor

Robot Integration

New Automation Capabilities for Centro and Mithras Microplate Readers

in-vivo Imaging

NEW - Trans-illumination – a Solution for Excitation of GFP Transfected Plants in Petri Dishes for In Vivo Imaging
Luciferin Bioavailability in Mice during in-vivo Imaging
Bioluminescence Imaging using NightOWL
Monitoring of Renilla Luciferase Activities in-vitro and in-vivo
Quantum dots in molecular imaging

Gel Documentation & Gel Imaging

Nucleic Acid Staining with GelStar®
Coomassie Brillant Blue Staining
Deep Purple Staining
SYPRO Ruby Staining
Dye Detection in Gel Imaging Systems

Radio flow through measurements using UPLC

Faster analysis of radio pharmaceuticals using UPLC™







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